Table 1 Multiple Primer Sequences used for assay optimization
Primer type | Primer name | Sequence (5′-3′) |
|---|---|---|
Deletion-specific primer | F1 | TTCTCTAGACCTGCTGCCTATACAG |
R1 | CCATAACAGGTCTTCATATTTCCGG | |
F2 | TCTCTAGACCTGCTGCCTATACCGT | |
R2 | CCATGCCCATAACAGGTCTTCATAT | |
F3 | AAGGAGAGGAGAGAGACTCAAGCAC | |
R3 | GCGAAACCACATAAAAGTGACTGTATAG | |
F4 | AAGGAGAGGAGAGAGACTCAAGCAC | |
R4 | AACCACATAAAAGTGACTGTATCGG | |
Insertion-specific primer | F1 | CTGGAGAGCCACTCCCATCCTTTCT |
R1 | TCGAGACCATCCCGGCTAAAAC | |
F2 | GCCACTCCCATCCTTTCT | |
R2 | CCTGTAATCCCAGCACTTTG |