From: Mutational Analysis of Ocriplasmin to Reduce Proteolytic and Autolytic Activity in Pichia pastoris
Ocriplasmin variants Kinetic parameters | Wild | Proteolytic | Autolytic | Mixed |
---|---|---|---|---|
Vmax | 0.123 | 0.0784 | 0.092 | 0.1081 |
Km | 908.4 | 906.7 | 989.8 | 1704 |
Kcat | 12.28 | 7.836 | 9.217 | 10.81 |
Kcat/Km | 0.0135 | 0.0086 | 0.0093 | 0.0063 |