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Table 2 kinetic parameters of the wild-type and mutant variants of ocriplasmin with the substrate S-2403

From: Mutational Analysis of Ocriplasmin to Reduce Proteolytic and Autolytic Activity in Pichia pastoris

Ocriplasmin variants Kinetic parameters

Wild

Proteolytic

Autolytic

Mixed

Vmax

0.123

0.0784

0.092

0.1081

Km

908.4

906.7

989.8

1704

Kcat

12.28

7.836

9.217

10.81

Kcat/Km

0.0135

0.0086

0.0093

0.0063