Table 1 The sequences of the primers used for amplification of Ocriplasmin variants
From: Mutational Analysis of Ocriplasmin to Reduce Proteolytic and Autolytic Activity in Pichia pastoris
Variants | 5′ sequence 3′ |
|---|---|
Wild | α factor-F1: CTATTGCCAGATTGCTGC CAC1-R1: GCGTGAATGTAAGCGTGAC |
Proteolytic | OCR-F2: GGTGCTGGTTTGTTGGAAGAAGCTCAATTGC OCR-R2: CAATTGAGCTTCTTCCAACAAACCAGCACCG |
Autolytic | OCR-F3: AGAATGGGTTTTGACTACTGCTCACTGTTTGG OCR-R3: CCAAACAGTGAGCAGTAGTCAAAACCCATTCT |
Mixed | OCR-F4: CGGTGCTGGTTTGTTGGAAGAAGCTCAATTGC OCR-R4: GCAATTGAGCTTCTTCCAACAAACCAGCACCG |