Fig. 5
From: Mutational Analysis of Ocriplasmin to Reduce Proteolytic and Autolytic Activity in Pichia pastoris
Activation of ocriplasmin by urokinase. Reducing SDS–PAGE illustrating that activation with urokinase is almost complete within 30 min. Ocriplasmin (27 KD) is converted into two fragments upon activation. Column 1: Protein marker, Column 2: Ocriplasmin before activation, Column 3–5: Samples collected 10, 30 and 60 min after addition of 2% urokinase. Column 6–8: Samples collected 10, 30 and 60 min after addition of 5% urokinase