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Fig. 4 | Biological Procedures Online

Fig. 4

From: Mutational Analysis of Ocriplasmin to Reduce Proteolytic and Autolytic Activity in Pichia pastoris

Fig. 4

Expression and characterization of ocriplasmin variants in P. pastoris. a Expression of ocriplasmin after optimization in P. pastoris. Column 1: Protein marker, Column 2: Negative control, (P. pastoris none transformed) Column 3–6: a 27 KDa protein product relating wild-type, autolytic, proteolytic and mixed variants, respectively. b Western blotting, Column 1: Protein marker, Column 2: Negative control, Column 3: Purified ocriplasmin (a 27 KDa protein product) expressed in P. pastoris. c Elisa assay using anti-plasminogen antibody and recombinant protein expressed in Pichia pastoris

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