Fig. 7
From: Development of a Novel Orthotopic Gastric Cancer Mouse Model
The procedure to transplant the homogeneous tissues under the stomach serosa layer. A. The layers compose the stomach wall. B. Using a 31 gauge needle on an insulin syringe 30–40 ul of either 50% matrigel diluted with RPMI media or sterile saline is injected into the subserosa area. C. The serosa over the injection site is incised 2–3 mm with Vannas Iridocapsulotomy Scissors in order to generate the entrance to insert the 18 gauge need and evacuate the space-creating 50% matrigel or saline. D. The solution which had leaked is wiped with sterile gauze or cotton swabs. E. A cutting site is widened using Micro-Blunted Tip Dumont Forceps or Moria Iris Forceps. F. An 18 gauge needle is placed into the inner-space. G. Fill the tissue slowly from the opposite area of the entrance. H. After implantation, the entry site is pressed lightly with a sterile cotton swab and the needle is gently removed. I. Giving an extremely light and gentle pressure the transplantation site to confirm the leaking and the entry site is pressed with a sterile cotton swab for 20–30 s, on average, until it closes completely. J. As an auxiliary closure, tissue adhesive is beneficial to cover the incision site of the stomach serosa