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Fig. 1 | Biological Procedures Online

Fig. 1

From: Development of a Novel Orthotopic Gastric Cancer Mouse Model

Fig. 1

The procedures for the completely-closed orthotopic transplantation of gastric cancer in NSG mice. A. a. Skin incision made using iris scissors. b. Exposing the stomach. Retractors hold both the skin and abdominal walls while forceps gently grab the stomach. B. A syringe assembly using an 18 gauge blunt needle and filled with homogenous minced tumor tissue. C. a. Injection of a low concentration of matrigel. b. Widening the entry to the space created under the serosa layer. c. Transplantation of up to 20ul of homogenous minced tissue. d. Tumor completely loaded into the stomach wall without leakage. D. a. Injection of a low concentration of matrigel. b. Widening the entry to the space created under the serosa layer. c. Transplantation of a 3 mm diameter fragment of soft tumor d. The tumor tissue completely inserted into the stomach wall without exposure. E. a. The abdominal wall closure with suture. b. The skin closed with wound clips. F. An illustration of the properly positioned transplantation site of the completely closed implantation method

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