Table 2 The radiosensitivity level of breast cancer patients, BRCA1/2 mutation carriers and breast cancer patients with radiotherapy complication, using G0/G2 chromosomal assay

 Ryabchenk et al. 2012 [148]

G0 and G2 chromosomal assay

1. BC cases (37)

Lymphocyte

G0 assay

G0 assay & G2 assay

2. Healthy controls (44)

1.5 Gy

Spontaneous RS

X-rays

BC cases ↔ Healthy controls

G2 assay

G0 assay & G2 assay

0.5 Gy

RS after radiation

X-rays

BC cases > Healthy controls

 Bryant et al. 2012 [151]

G2 chromosomal assay

1. BC cases (89)

Lymphocyte

0.4 Gy

RS after radiation

2. Healthy controls (96)

γ-rays

BC cases > Healthy controls

 Wang et al. (2012) [152]

G2 chromosomal assay

1. BC cases (515)

Lymphocyte

1.5 Gy

non-Hispanic White

2. Healthy controls (402)

γ-rays

BC cases > Healthy control

Mexican American

BC cases > Healthy control

African Americans

BC cases ↔ Healthy controls

 Poggioli et al. 2010 [150]

G0 & G2 chromosomal assay

1. BC cases (23)

Lymphocyte

G2 assay

G0 assay & G2 assay

2. Healthy controls (23)

0.4 Gy

Spontaneous RS

X-rays

BC cases ↔ Healthy controls

G0 assay

G0 assay & G2 assay

2 Gy

RS after radiation

X-rays

BC cases > Healthy controls

G2 assay could be more appropriate to define the individual RS if compare with G0 assay

 Howe et al. 2005 [149]

G2 chromosomal assay

1. BC cases (27)

Lymphocyte

0.5 Gy

RS after radiation

2. Healthy controls (14)

γ-rays

BC cases > Healthy controls

 Riches et al. 2001 [147]

G2 chromosomal assay

1. BC cases (65)

Lymphocyte

0.4 Gy

RS after radiation

2. Healthy controls (66)

γ-rays

BC cases > Healthy controls

 Scott et al. 1999 [135]

G2 chromosomal assay

1. BC cases (135)

Lymphocyte

0.5 Gy

Spontaneous RS

2. Healthy controls (105)

X-rays

Healthy control ↔ BC cases

RS after radiation

BC cases > Healthy controls

 Ernestos et al. 2010 [153]

G2 chromosomal assay

1. BC cases with BRCA1/2 mutation (15)

Lymphocyte

1 Gy

RS after radiation

2. Healthy BRCA1/2 mutation carriers (5)

γ-rays

BC with BRCA1/2 mutation > Healthy controls

3. Healthy controls W/O familial history of cancer (21)

Healthy BRCA1/2 mutation carriers > Healthy controls

 Baeyens et al (2002) [139]

G2 chromosomal assay

1. BC cases with BRCA1/2 mutation (20)

Lymphocyte

0.4 Gy

Spontaneous RS

 A. BRCA1(11)

γ-rays

No difference across all groups

 B. BRCA2 (9)

RS after radiation

2. Healthy relatives with BRCA mutation (12)

BC with BRCA1 mutation ↔ BC W/O BRCA mutation ↔ healthy controls

 A. BRCA1 (6)

BC with BRCA2 mutation > healthy controls

 B. BRCA2 (6)

Healthy relatives with and W/O a BRCA1 mutation ↔ healthy controls

3. Healthy relatives W/O BRCA mutation (10)

 A. of BRCA1 (5)

 B. of BRCA2 (5)

4. BC cases W/O BRCA mutation (78)

5. Healthy controls (58)

 Finnon et al. 2012 [143]

G2 chromosomal assay

1. BC cases with marked reaction (31)

Lymphocyte

3.5 Gy

RS after radiation

2. BC cases with mild late adverse reaction (28)

X-rays

Marked reaction ↔ Mild late adverse reaction

 Barber et al. 2000 [134]

G2 chromosomal assay

HDR

Acute reactions before radiotherapy (116)

Late reactions, 8-14 years after radiotherapy (47)

LDR

Acute reactions before radiotherapy (73)

Late reactions, 8-14 years after radiotherapy (26)

Lymphocyte

HDR

HDR

3.5 Gy γ-rays (dose rate 1.0 Gy min-1)

RS after radiation

Acute reactions before radiotherapy ↔ 8-14 years after radiotherapy

LDR

3.5 Gy γ-rays (dose rate 0.15 Gy min-1)

LDR

RS after radiation

Acute reactions before radiotherapy ↔ 8-14 years after radiotherapy