Fig. 5

Selection strategy for the apoptotic bodies population and determination of the recovery efficiency on the isolation process. Construction of the apoptotic bodies gate on the log-FSC-SSC plot using size calibrated-fluorescent beads are depicted (top). Representative flow cytometry plot of vesicles sample (previously gated on size selection) analyzed for the expression of apoptotic bodies markers using Annexin V-Dy-634/propidium iodide (PI) staining are shown (a). The apoptotic bodies (ApB) concentration of the unprocessed plasma samples (filled black symbols) and the isolated vesicles preparations (empty pink symbols) from patients with ischemic stroke (n = 4), multiple sclerosis (n = 4) and Parkinson’s disease (n = 4) and controls (n = 6) are represented (b). Representative analysis showing AnnexinV-Dy-634⁺PI⁺ apoptotic bodies (ApB) (pink points) on the FSC-SSC plots of plasma samples (left) and isolated vesicles (ApB) preparations (right) from neurological patients and healthy volunteers. The percentage yield of the isolation protocol, calculated by dividing the amount of isolated apoptotic bodies by the levels of these vesicles found in the starting plasma and multiply by 100, is indicated (c)