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Fig. 4 | Biological Procedures Online

Fig. 4

From: Bimodal Whole-Mount Imaging of Tendon Using Confocal Microscopy and X-ray Micro-Computed Tomography

Fig. 4

3D visualisation of LAMA4-immunolabelled equine superficial digital flexor tendon. 3D reconstructions of an equine superficial digital flexor tendon showing longitudinal nuclear (blue; a-c) and LAMA4 (red; e-g) labelling, and with each channel overlaid to create a composite image (i-k) rotated at x = − 45°, 0°, + 45°. Transverse (xz) views of 3D reconstructions of equine SDFT (d,h,l) demonstrate signal present throughout the depth of the tissue. Interfascicular matrix (IFM) and fascicles (f) are denoted longitudinally (b) and transversely (d). Scale bars = 200 µm

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