Fig. 5

The protein rank of sEVs-associated proteins and common contaminants identified by mass spectrometry. All proteins were ranked by intensity based absolute quantitation (iBAQ) values detected by mass spectrometry, which directly reflected the protein abundance. a The diverse rank of sEVs-associated proteins in the isolates of UC, SEC, combining SEC with UC methods. The dot plot highlighted several common sEVs-associated proteins rank, including CD9, CD81, ITGB1, HSP90AA1, RAB27B, CD63, ALIX, and FLOT1. b Diverse sEVs contaminants of plasma lipoproteins and other free-floating proteins rank in the isolates of three methods. The dot plot highlighted proteins rank, including APOE, APOL1, APOA1, APOA2, IgG L chain, and IgG H chain. c The diagram showed the protein rank of 8 sEVs-associated proteins in total detected proteins among UC, SEC, combining SEC with UC. Top 0% represents the protein expression is the highest in MS-analysis, and Top 100% means the lowest. Data not shown of undetected proteins. d The diagram showed the protein rank of 4 lipoproteins and 2 IgG fractions in total detected proteins among UC, SEC, combining SEC with UC. Rank 0 represents the protein expression is the highest in MS-analysis