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Fig. 4 | Biological Procedures Online

Fig. 4

From: Combination of Size-Exclusion Chromatography and Ultracentrifugation Improves the Proteomic Profiling of Plasma-Derived Small Extracellular Vesicles

Fig. 4

Proteomic analysis of sEVs isolated by UC, SEC, and combining SEC with UC. a The total protein species of plasma sEVs isolated by three methods were determined by mass spectrometry-based analysis. UC isolates (protein species: 453), SEC isolates (protein species: 682), combining SEC with UC isolates (protein species: 992). b The heatmap showed all detected proteins’ abundance of plasma sEVs isolated by SEC, combining SEC with UC (SEC + UC), and UC. c As compared to SEC, there were 345 proteins only identified in the plasma sEVs isolates of the combination method (SEC + UC). GO/KEGG enrichment was carried to analyze these proteins identified by the combination method but not by SEC (left: a bubble plot of GOs; right: a bubble plot of 21 KEGG pathways enriched). d As compared to UC, there were 591 proteins only identified in the plasma sEVs isolates of the combination method (SEC + UC). GO/KEGG enrichment was performed to analyze these proteins identified by the combination method but not by UC (left: a bubble plot of GOs; right: a bubble plot of 46 KEGG pathways enriched). e-g Bioinformatic analysis exhibited the sEVs-associated proteins species and numbers of plasma sEVs. In total, at the intersecting area of Venn diagram, 180 sEVs-associated proteins were identified by using UC, 366 sEVs-associated proteins by using SEC, and 584 sEVs-associated proteins by using SEC and UC

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