Fig. 3

Particle number and protein content of plasma sEVs isolated by UC, SEC, SEC and UC. a The particle number of isolated fractions by using UC, SEC, combining SEC and UC was measured by NTA (**p < 0.01, ***p < 0.001; n = 3 for each group). b The total protein content of isolated particles in three groups was measured by BCA assay (*p < 0.05, **p < 0.01; n = 3 for each group). c The particle number/fg protein ratio for three groups (***p < 0.001; n = 3 for each group). d Western blotting showed the expression of two common contaminations (Albumin and Apo-A1) and one negative marker of sEVs (Calnexin) on the vesicles isolated by UC, SEC, combining SEC with UC