Fig. 7

a–d qRT-PCR with Caco-2 cells for ABCA1, ABCG5, NPC1L1 and ABCB1. Caco-2 cells cultivated on filter inserts were treated for the indicated time points with bexarotene (RXR agonist, 5 μM), GW3965 (LXR agonist, 10 μM) or the vehicle control (DMSO 0.1%). Extraction of RNA and quantification of mRNA expression by qRT-PCR was carried out as described in Supplementary Protocol #6 (Additional file 1). Results were expressed as the ratio of expression of the respective gene to that of GAPDH and were then normalized to the vehicle control of the respective time point (vehicle control not indicated for each time point). Bar graphs represent mean ± SD from three to four independent experiments. *P < 0.05, **P < 0.01 versus solvent vehicle (determined by paired t-test)