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Fig. 6 | Biological Procedures Online

Fig. 6

From: High-Resolution Confocal Fluorescence Imaging of Serine Hydrolase Activity in Cryosections – Application to Glioma Brain Unveils Activity Hotspots Originating from Tumor-Associated Neutrophils

Fig. 6

High-resolution imaging of TAMRA-FP hotspots and TAMRA-FP hotspot clusters and their inhibitor sensitivity in glioma. In a, the upper panel shows hematoxin-eosin (H&E) stained section. For images in the lower panel, sections went through the tissue-ABPP protocol to label SHs (red), followed by DAPI staining to visualize nuclei (blue). Note presence of multi-nucleated cells with TAN-like morphology in regions of TAMRA-FP hotspots and TAMRA-FP hotspot clusters. In b, sections were pretreated with DMSO or with the SH inhibitors PMSF (1 mM) or deshiobiotin-FP (DeBi-FP, 5 μM) for 1 h at RT, after which they went through the tissue-ABPP protocol to label SHs (red), followed by DAPI staining to visualize nuclei (blue). Note that throughout the examined regions, TAMRA-FP labeling is sensitive to the inhibitors. Scale bars 10 μm (a) and 20 μm (b). Images were adjusted for brightness and contrast

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