Fig. 5

Positions of spheroids in matrices and subsequent cell migration. Schematic diagrams on the left panel indicate corresponding spheroids position of the fluorescent image on the right panel. The images show an x-z view of LLC cells migrating in collagen I. a Spheroids sediment at the bottom in matrix and cells tend to follow the interface between gel and bottom surface. b A bottom layer of gel was made in the culture plate before adding matrix with spheroids. Most cells move along the interface between the two layers of gel. c To prevent spheroids settling, 96-well plate was inverted for 1–2 min at room temperature and spheroids may stay in matrix or near to top. d A representative picture of cell dispersing when spheroids are in a proper position of a homogenous collagen I gel