Fig. 4

Migration index shows cell invasive capacity. Fluorescently labeled LLC cells were used for invasion test in this 3D assay to compare the effect of 20% FBS vs. 10% FBS. a Representative pictures of LLC cell dispersion at 72 h. Cell were color coded for analysis after running additional macros in Fiji. Red circles show microcarrier beads in spheroids, and green areas show distribution of migrating cells at 72 h. b Line graph shows migration area changes based on the distance to core at T = 72 h. c Calculation of migration index using data of each time point. Data represent mean ± standard deviation (N = 3). NS, not significant