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Fig. 3 | Biological Procedures Online

Fig. 3

From: A microcarrier-based spheroid 3D invasion assay to monitor dynamic cell movement in extracellular matrix

Fig. 3

Content and concentration of matrices influence cell invasion. a Lewis lung carcinoma (LLC) cells were red fluorescently labeled in cytoplasm and green fluorescently labeled in nucleus. Cell coated microcarrier beads were embedded in 5 mg/ml growth factor reduced (GFR) Matrigel, 1.6 mg/ml collagen I or 0.3% agar respectively, and pictures were taken 56 h later. Scale bars, 100 μm. b Melanoma cell line M14 were grown on beads and cell invasion was monitored in a series of concentrations of collagen I gel. Five spheroids were recorded for each individual assay and migratory distance was measured in three independent experiments. Error bars represent standard deviation. c Representative pictures of M14 invasion in different concentration of collagen I for 6 days. Scale bar, 100 μm

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