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Table 1 Physicochemical characteristics of fractions I, II and III of total GAGs

From: Glycosaminoglycans from Co-Products of «Scyliorhinus canicula»: Extraction and Purification in Reference to the European Pharmacopoeia Requirement

TESTS

TCA

ENZ.DIG

ALKA.HYDRO

ASPECT

SOLUBILITY

WEIGHT (g)

YIELD (%)

pH

L.O.D (%)

SODIUM.R

CHLORIDE.TEST

Fractions

FRACTION I

+

+

Wh.Hyg.Pow

Wat.Solu

Act/Eth.Ins

1.35

6.75

6.65

10.8

COMPLIANT

COMPLIANT

FRACTION II

+

Wh.Hyg.Pow

Wat.Solu

Act/Eth.Ins

3.25

16.25

6.68

COMPLIANT

COMPLIANT

FRACTION III

+

+

Wh.Hyg.Pow

Wat.Solu

Act/Eth.Ins

3.35

16.75

6.81

COMPLIANT

COMPLIANT

  1. (+): added
  2. (−): not added
  3. ENZ.DIG Enzymatic digestion
  4. ALKA.HYDRO Alkaline hydrolysis
  5. Trichloroacetic acid deproteinisation
  6. Wh.Hyg.Pow white hygroscopic powder
  7. Wat.Solu easily soluble in water
  8. Act/Eth.Ins Insoluble in acetone and 96% ethanol
  9. L.O.D loss on drying
  10. SODIUM.R sodium reaction