Fig. 4

AKT1 over-expression promoted cytokines release by MSCs. a The mRNA expression of IL-4, IL-10, PTGES2, HGF and VEGF in Null-MSCs and AKT1-MSCs were measured by qRT-PCR under routine culture condition and IFN-γ stimulation condition. The gene transcript levels were normalized to those of GAPDH and set to 1 in Null-MSCs untreated group. b ELISA analysis for detecting IL-10, PGE2, VEGF and HGF protein expression in MSCs culture supernatant after IFN-γ stimulation for 24h. The data are presented as the means ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001