Fig. 2

Over-expression of AKT1 in MSCs. a At 72h after infection, the transduction efficiency of Null-MSCs and AKT1-MSCs was evaluated by fluorescence microscopy (left, Scale bar = 200μm) and flow cytometry (right). Non-infected cells were used as the control for setting gates. b At 72h after infection, the infected cells were sorted by flow cytometry for GFP+ population. The mRNA expression levels of AKT1 were quantified by qRT-PCR, normalized to those of GAPDH and set to 1 in Null-MSCs group. The data are presented as the means ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. C. Representative western blots showing total AKT1 and phosphorylated AKT1 expression in Null-MSCs and AKT1-MSCs