Fig. 5

Multiplexed XBP1 assessment following inflammasome activation. THP-1 cells were treated with LPS (1 μg/ml, 4 h) followed by nigericin (10 μM, 45 min), resulting in NLRP3 inflammasome activation and an increase in XBP1 splicing. Immunoblot of IL-1β in the conditioned media confirmed its release and inflammasome activation (a). XBP1 transcript and protein levels were assessed by RT-PCR (a) and biochip (b) respectively. * p < 0.05