Fig. 4
BATTM technology detection of XBP1s and XBP1u levels in U937 lysates. 2.5X106 untreated and Tg (0.25 mM, 48 h) treated U937 cells were lysed in RIPA and immunoblotted for XBP1s and XBP1u alongside a serial dilution of His-tagged recombinant protein. β-Actin acts as a loading control (a). RNA was extracted from the same cells and analysed by RT-PCR for XBP1 spicing. GAPDH serves as a control (b). RIPA lysed U937 cells were treated as in (a) and assessed by XBP1 biochip. ** p < 0.01 (c)