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Fig. 2 | Biological Procedures Online

Fig. 2

From: Manipulating osa-MIR156f Expression by D18 Promoter to Regulate Plant Architecture and Yield Traits both in Seasonal and Ratooning Rice

Fig. 2

Expression of osa-MIR156f in transgenic rice. a Phenotype of transgenic rice lines including three D18p::osa-MIR156f lines (D18–3, D18–8 and D18–11) and three UBQpro::osa-MIR156f lines (U-1, U-16 and U-19). b Expression analysis of pri-miR156f in different tissues of D18–11 and the wild type (WT) rice at the heading stage. c Expression analysis of pri-miR156f in different tissues of D18–11 and the WT rice at 3-week-old seedling stage. d Mature osa-miR156 level in different tissues of D18–11 and WT rice at 3-week-old seedling stage. The ΔΔCt method was used to calculate the relative gene expression. Error bars indicated the standard deviation (SD). Three independent biological replicates were analyzed (n = 5). Asterisks indicate a significant difference to WT rice based on a t test. *: significant difference compared to WT rice at a 5% level (P < 0.05); **: significant difference compared to WT rice at a 1% level (P < 0.01)

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