Fig. 1

Expression pattern of osa-MIR156f. a The typical hairpin structure of pre-miR156f and the 20 nt of the mature miR156f. b Localization of GUS activity driven by the osa-MIR156f native promoter in different tissues. The sampling dates for tissues were as follows: shoot apex at the 3-week-old seedling stage, stem at the flowering stage, axillary bud at the heading stage, root at the 3-week-old seedling stage, root hair at the tillering stage, young spike at the heading stage, young glumous flower at the panicle booting stage, leaf segment at the heading stage and stamen at the flowering stage. c Relative expression of pri-miR156f in various rice tissues or organs: 1, leaf lamina at tillering stage; 2, leaf sheath at the tillering stage; 3, shoot apex at the tillering stage; 4, stem segment (about 0.5 cm) above the node at the flowering stage; 5, axillary bud at the tillering stage; 6, young spike at the heading stage; 7, young glumous flower at the heading stage; 8, stamen (ND: not detected); 9, basal node at the heading stage; 10, root tip (about 0.5 cm) at the heading stage; 11, 3-week-old leaf; 12, 3-week-old shoot; 13, 3-week-old basal node; 14, 3-week-old root. The ΔΔCt method was used to calculate the relative gene expression. Error bars indicated the standard deviation (SD). Three independent biological replicates were analyzed (n = 3)