Table 3 Reaction system for demodification by CobB TIMING: 0.5 h
From: Cell-Wide Survey of Amide-Bonded Lysine Modifications by Using Deacetylase CobB
The demodification reactions were carried out in a 600 μl reaction mix contains: | |
|---|---|
Reagent name | Amount |
pH 7.5 HEPES buffer (500 mM) | 60 μl |
DTT (100 mM) | 6 μl |
MgCl2 (600 mM) | 6 μl |
NAD+ (100 mM) | 6 μl |
Substrate proteome (10 μg/ml) | 300 μl |
CobB (5 mg/ml) | 50 μl |
PMSF (100 mM) | 0.3 μl |
ddH2O | Up to 600 μl |