Fig. 3
From: Cell-Wide Survey of Amide-Bonded Lysine Modifications by Using Deacetylase CobB
An example flow chat of how a positive identification (asparagine) was obtained. A GC peak at certain retention time (19.498 min) is generated by CobB treatment (1), the MS/MS spectrum of the 19.498 min peak generated by CobB treatment (2), Searching against NIST library identified that the metabolite of 19.498 min peak was asparagine (3), the standard asparagine generate the same GC retention time and MS/MS spectrum as the metabolite generated by CobB treatment (4)