Table 4 Summary of qMSP methylation results calculated using demethylation index and ΔΔCt approach
From: DNA Methylation Validation Methods: a Coherent Review with Practical Comparison
Locus and used primer set | Demethylation index | ΔΔCt | ||
|---|---|---|---|---|
Average (n = 10) | ± SD | Average (n = 10) | ± SD | |
M Met | 2.02 | 0.60 | 2.09 | 0.66 |
IM Met | 0.05 | 0.03 | 0.03 | 0.02 |
IM Unm | 0.51 | 0.10 | 0.50 | 0.12 |
U Unm | 1.03 | 0.30 | 1.30 | 0.66 |