Fig. 3
From: DNA Methylation Validation Methods: a Coherent Review with Practical Comparison
Positions of primer pairs, CpGs and restriction sites within studied regions. CpGs are shown as red and yellow bars on a line representing the DNA sequence. The red CpG is the one originally chosen from Infinium MethylationEPIC BeadChip. The scissors indicate sites that are cut by MSREs. The lighter blue primers were used for initial pyrosequencing PCR and the darker blue primers represent the sequencing primers. The patterned light green HRM primers were designed with a CpG on its 5’end (M/U Wojdacz primers)