Fig. 2
From: DNA Methylation Validation Methods: a Coherent Review with Practical Comparison
Summary of DNA methylation levels measured by the investigated methods. The average methylation of 10 samples is shown, the error bars represent the SD. Displayed MSRE data were measured after 2-h digestion. Displayed MS-HRM data were acquired using HRM M, HRM IM and HRM U Wojdacz primers. qMSP data shown were calculated using ΔΔCt approach and multiplied by 100 to gain the percentage. For the M locus in qMSP, all values were higher than 100% so we set the mean to 100% to make the figure more comprehensible, the SD was calculated from the original values multiplied by 100. For the IM and U loci in qMSP, we calculated the methylation percentage as 1-(Unm ΔΔCt), the SD was also calculated from the original ΔΔCt values multiplied by 100. M – methylated locus, IM – intermediately methylated locus, U – unmethylated locus