From: Development and Validation of Quantitative Real-Time PCR for the Detection of Residual CHO Host Cell DNA and Optimization of Sample Pretreatment Method in Biopharmaceutical Products
Results
Valid method range
3 fg/ul-300 pg/ul
Linearity
1.000
LOD
3 fg/ul
LOQ
0.3 pg/reaction
Accuracy
82.3–105.7%
Precision
Repeatability(intra-precision)
0.065–0.452%
Inter- precision
0.471–1.312%