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Table 6 Accuracy test by observing recovery of different spike concentrations

From: Development and Validation of Quantitative Real-Time PCR for the Detection of Residual CHO Host Cell DNA and Optimization of Sample Pretreatment Method in Biopharmaceutical Products

Spiking concentration(pg/ml)

Measured(pg/ml)

Average recovery(%)

CV(%)

600

494 ± 19.8

82.3

4.0

200

206.7 ± 7.9

103.3

3.8

20

21.1 ± 4.5

105.7

21