From: Development and Validation of Quantitative Real-Time PCR for the Detection of Residual CHO Host Cell DNA and Optimization of Sample Pretreatment Method in Biopharmaceutical Products
Spiking concentration(pg/ml)
Measured(pg/ml)
Average recovery(%)
CV(%)
600
494 ± 19.8
82.3
4.0
200
206.7 ± 7.9
103.3
3.8
20
21.1 ± 4.5
105.7
21