Development and Validation of Quantitative Real-Time PCR for the Detection of Residual CHO Host Cell DNA and Optimization of Sample Pretreatment Method in Biopharmaceutical Products

Table 5 Inter-assay precision(intermediate precision)test

Standard(pg/ml)	Mean C_T			Standard deviation	C.V.(%)
Standard(pg/ml)	Day1	Day2	Day3	Standard deviation	C.V.(%)
300	14.50	14.3	14.73	0.190	1.312
30	17.78	17.53	17.9	0.179	1.011
3	21.41	21.12	21.49	0.165	0.772
0.3	25.05	24.83	25.00	0.118	0.471
0.03	28.5	28.21	28.42	0.141	0.497
0.003	32.09	31.76	31.82	0.188	0.591

ISSN: 1480-9222