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Table 3 Limit of quantification(LOQ)test

From: Development and Validation of Quantitative Real-Time PCR for the Detection of Residual CHO Host Cell DNA and Optimization of Sample Pretreatment Method in Biopharmaceutical Products

Spike amount (pg/reaction)

Mean CT*

Mean value of observed DNA (pg/reaction)

Standard deviation

Average Recovery(%)

3

31.98

3.14

0.298

105

0.3

35.31

0.294

0.173

98

  1. *CT, Cycle Threshold
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