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Table 1 Comparison of detected concentration after standardization

From: Development and Validation of Quantitative Real-Time PCR for the Detection of Residual CHO Host Cell DNA and Optimization of Sample Pretreatment Method in Biopharmaceutical Products

 

observed concentration(pg/ml)

Recovery(%)

Standardized DNA concentration(pg/ml)

Kit

0.337

0.51

0.365

83.3

0.485

Alu-primer/probe

0.331

0.478

0.485

74.7

0.577