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Table 3 Determination of the appropriate cocktail solution containing hemolysates and biotin-PL3 and HRP-streptavidin

From: Immunostick Test for Detecting ζ-Globin Chains and Screening of the Southeast Asian α-Thalassemia 1 Deletion

Cocktail mixtures Hb Bart’s hydrops fetalis hemolysates (containing 5 μg/mL of ζ-globin) Normal hemolysates dilution 1:500
Test paddle Internal control paddles Test paddle Internal control paddles
PL3-biotin (μg/mL) Streptavidin-HRP (dilution) Positive control Negative control Positive control Negative control
Isotype matched control Uncoat cpntrol Isotype matched control Uncoat control
0.125 1:500 DBb DB LBa Nc LB DB LB N
0.250 DB DB LB N LB DB LB N
0.500 DB DB LB N LB DB LB N
0.125 1:1000 DB DB N N N DB N N
0.250 DB DB N N N DB N N
0.500 DB DB LB N LB DB LB N
0.125 1:2000 LB DB N N N DB N N
0.250 LB DB N N N DB N N
0.500 LB DB N N N DB N N
  1. aLight blue: Weakly positive reactivity
  2. bDark blue: Strongly positive reactivity
  3. cNo color: Negative reactivity