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Table 3 Determination of the appropriate cocktail solution containing hemolysates and biotin-PL3 and HRP-streptavidin

From: Immunostick Test for Detecting ζ-Globin Chains and Screening of the Southeast Asian α-Thalassemia 1 Deletion

Cocktail mixtures		Hb Bart’s hydrops fetalis hemolysates (containing 5 μg/mL of ζ-globin)				Normal hemolysates dilution 1:500
Cocktail mixtures		Test paddle	Internal control paddles			Test paddle	Internal control paddles
PL3-biotin (μg/mL)	Streptavidin-HRP (dilution)		Positive control	Negative control			Positive control	Negative control
PL3-biotin (μg/mL)	Streptavidin-HRP (dilution)		Positive control	Isotype matched control	Uncoat cpntrol		Positive control	Isotype matched control	Uncoat control
0.125	1:500	DB^b	DB	LB^a	N^c	LB	DB	LB	N
0.250		DB	DB	LB	N	LB	DB	LB	N
0.500		DB	DB	LB	N	LB	DB	LB	N
0.125	1:1000	DB	DB	N	N	N	DB	N	N
0.250		DB	DB	N	N	N	DB	N	N
0.500		DB	DB	LB	N	LB	DB	LB	N
0.125	1:2000	LB	DB	N	N	N	DB	N	N
0.250		LB	DB	N	N	N	DB	N	N
0.500		LB	DB	N	N	N	DB	N	N

^aLight blue: Weakly positive reactivity
^bDark blue: Strongly positive reactivity
^cNo color: Negative reactivity

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ISSN: 1480-9222

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