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Table 2 Determination of optimal dilution of hemolysates

From: Immunostick Test for Detecting ζ-Globin Chains and Screening of the Southeast Asian α-Thalassemia 1 Deletion

Sample number Hemolysates of non-SEA type α-thalassemia 1
Dilution 1:250 Dilution 1:500 Dilution 1:1000
Test paddle Internal control paddles Test paddle Internal control paddles Test paddle Internal control paddles
Positive control Negative control Positive control Negative control Positive control Negative control
Isotype matched control Uncoat control Isotype matched control Uncoat control Isotype matched control Uncoat control
N1 LBa DBb LB Nc N DB N N N DB N N
N2 N DB N N N DB N N N DB N N
N3 LB DB LB N N DB N N N DB N N
N4 LB DB LB N N DB N N N DB N N
N5 LB DB LB N N DB N N N DB N N
N6 N DB N N N DB N N N DB N N
N7 LB DB N N N DB N N N DB N N
N8 N DB N N N DB N N N DB N N
N9 LB DB LB N N DB N N N DB N N
N10 N DB N N N DB N N N DB N N
  1. aLight blue: Weakly positive reactivity
  2. bDark blue: Strong positive reactivity
  3. cNo color: Negative reactivity