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Table 2 Determination of optimal dilution of hemolysates

From: Immunostick Test for Detecting ζ-Globin Chains and Screening of the Southeast Asian α-Thalassemia 1 Deletion

Sample number

Hemolysates of non-SEA type α-thalassemia 1

Dilution 1:250

Dilution 1:500

Dilution 1:1000

Test paddle

Internal control paddles

Test paddle

Internal control paddles

Test paddle

Internal control paddles

Positive control

Negative control

Positive control

Negative control

Positive control

Negative control

Isotype matched control

Uncoat control

Isotype matched control

Uncoat control

Isotype matched control

Uncoat control

N1

LBa

DBb

LB

Nc

N

DB

N

N

N

DB

N

N

N2

N

DB

N

N

N

DB

N

N

N

DB

N

N

N3

LB

DB

LB

N

N

DB

N

N

N

DB

N

N

N4

LB

DB

LB

N

N

DB

N

N

N

DB

N

N

N5

LB

DB

LB

N

N

DB

N

N

N

DB

N

N

N6

N

DB

N

N

N

DB

N

N

N

DB

N

N

N7

LB

DB

N

N

N

DB

N

N

N

DB

N

N

N8

N

DB

N

N

N

DB

N

N

N

DB

N

N

N9

LB

DB

LB

N

N

DB

N

N

N

DB

N

N

N10

N

DB

N

N

N

DB

N

N

N

DB

N

N

  1. aLight blue: Weakly positive reactivity
  2. bDark blue: Strong positive reactivity
  3. cNo color: Negative reactivity
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