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Table 4 Responses of single copy sinI-uidA transcriptional fusion to expR sinI, sinI, expR, and AHL in medium TY

From: Use of Plasmid pVMG to Make Transcriptional ß-Glucuronidase Reporter Gene Fusions in the Rhizobium Genome for Monitoring the Expression of Rhizobial Genes In Vivo

Strain

Genotype

ß-glucuronidase activitya,b

8530 sinI::VMG495

expR+, sinI+

194 ± 4

MG32 sinI::VMG495

expR+, sinI-

33 ± 3

MG32 sinI::VMG495 (C16:1)

expR+, sinI-

192 ± 2

1021 sinI::VMG495

expR-, sinI+

95 ± 3

8530 nop::VMG209

expR+, sinI+

<  0.1

  1. aNanomoles per minute per OD595 [15].
  2. bShown are averages and standard deviations (n = 3).
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