Table 3 Primers used in the study. Sal I and BamH I restriction sites are underlined.
Name | Sequence (5′-3′) | usage |
|---|---|---|
Primer 1-sinI | ACGCGTCGACGTTGAGTGGTCCGCCTACCG | sinI forward |
Primer 2-sinI | GCTGCGACCGGATCCGTTCACTAT | sinI reverse |
Primer 3-sinI | GGTGGAATGGGCGACAGCGCG | sinI forward |
Primer 4 | GGGTTGGGGTTTCTACAGGA | uidA reverse |
Primer 1-nop | AGCCTTGAACGTCGACTG | nop forward |
Primer 2-nop | ATGGAGGATCCAGCGAG | nop reverse |
Primer 3-nop | AAGTTGGGGTATCGCCCTAAA | nop forward |