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Table 1 Strains and plasmids

From: Use of Plasmid pVMG to Make Transcriptional ß-Glucuronidase Reporter Gene Fusions in the Rhizobium Genome for Monitoring the Expression of Rhizobial Genes In Vivo

Strain or plasmid Relevant features Source or Reference
Strains
 DH5a a-complementation Invitrogen
 8530 S. meliloti, expR+, sinI+, Smr [10]
 1021 S. meliloti, expR-, sinI+, Smr [11]
 MG32 8530 with sinI deletion expR+, sinI, Smr [12]
 8530 sinI::VMG495 sinI-uidA transcriptional fusion, expR+, sinI+, Smr, Nmr This work
 1021 sinI::VMG495 sinI-uidA transcriptional fusion, expR+, sinI+, Sm, Nm This work
 MG32 sinI::VMG495 sinI-uidA transcriptional fusion, expR+, sinI-, Smr, Nmr This work
 8530 nop::VMG209 Non promoter (nop) DNA-uidA, expR+, sinI+, Smr, Nmr This work
Plasmids
 pVO155 pUC19-derived integrational uidA vector [1]
 pVMG pVO155 with stop codons upstream of promoterless uidA in all ORFs This work, [12]
 pRK600 pRK2013 Nm::Tn9, Cmr [1]
 pVMG495 pVMG, sinI 5’-end, transcriptional fusion This work
 pVMG209 pVMG, nop DNA of S. meliloti. This work
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