Use of Plasmid pVMG to Make Transcriptional ß-Glucuronidase Reporter Gene Fusions in the Rhizobium Genome for Monitoring the Expression of Rhizobial Genes In Vivo

Table 1 Strains and plasmids

Strain or plasmid	Relevant features	Source or Reference
Strains
DH5a	a-complementation	Invitrogen
8530	S. meliloti, expR+, sinI+, Sm^r	[10]
1021	S. meliloti, expR-, sinI+, Sm^r	[11]
MG32	8530 with sinI deletion expR+, sinI⁻, Sm^r	[12]
8530 sinI::VMG495	sinI-uidA transcriptional fusion, expR+, sinI+, Sm^r, Nm^r	This work
1021 sinI::VMG495	sinI-uidA transcriptional fusion, expR+, sinI+, Sm, Nm	This work
MG32 sinI::VMG495	sinI-uidA transcriptional fusion, expR+, sinI-, Sm^r, Nm^r	This work
8530 nop::VMG209	Non promoter (nop) DNA-uidA, expR+, sinI+, Sm^r, Nm^r	This work
Plasmids
pVO155	pUC19-derived integrational uidA vector	[1]
pVMG	pVO155 with stop codons upstream of promoterless uidA in all ORFs	This work, [12]
pRK600	pRK2013 Nm::Tn9, Cm^r	[1]
pVMG495	pVMG, sinI 5’-end, transcriptional fusion	This work
pVMG209	pVMG, nop DNA of S. meliloti.	This work

ISSN: 1480-9222