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Fig. 3 | Biological Procedures Online

Fig. 3

From: Use of Plasmid pVMG to Make Transcriptional ß-Glucuronidase Reporter Gene Fusions in the Rhizobium Genome for Monitoring the Expression of Rhizobial Genes In Vivo

Fig. 3

Histochemical assay of M. truncatula nodules. a 8530 sinI::VMG495 (n = 13) b 8530 nop::VMG209 (n = 5). Nodules were stained with 4 mg /ml X-Glue in NaH2PO4. pH 7.2, 37 °C as described [26] for 3 h (a) or for 48 h (b). Photographs were taken 7 weeks after inoculation with Olympus MVX10 dissecting scope equipped with a MicroFire camera (Optitronics, Goleta, CA, USA)

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