Fig. 3
Histochemical assay of M. truncatula nodules. a 8530 sinI::VMG495 (n = 13) b 8530 nop::VMG209 (n = 5). Nodules were stained with 4 mg /ml X-Glue in NaH2PO4. pH 7.2, 37 °C as described [26] for 3 h (a) or for 48 h (b). Photographs were taken 7 weeks after inoculation with Olympus MVX10 dissecting scope equipped with a MicroFire camera (Optitronics, Goleta, CA, USA)