Fig. 1

Structure and multiple cloning sites (MCS) of the plasmid pVMG. a. pVMG, a derivative of pVO155, contains a short DNA linker (red) for translation termination codons to ensure transcriptional fusions. The size of pVMG was deduced from the known sequence of the analogous plasmid pVMGTnpR (GenBank accession number EU232661). b. The MCS of pVMG. All the restriction sites are usable except the Xba I site. The Xba I is located after translation termination codons, therefore not readily usable for making transcriptional fusions