A Cautionary Tale: Endogenous Biotinylated Proteins and Exogenously-Introduced Protein A Cause Antibody-Independent Artefacts in Western Blot Studies of Brain-Derived Proteins

Table 1 Antibodies

Antibody	Format	Isotype	Vendor	Product Number
Anti-Aβ₄₀	Affinity-purified monoclonal	Mouse IgG1, κ	Gift of Dr. Pritam Das	13.1.1
Anti-Aβ₄₂	Affinity-purified monoclonal	Mouse IgG1, κ	Gift of Dr. Pritam Das	2.1.3
Anti-APP(C-terminal)	Affinity-purified monoclonal	Rabbit IgG	Invitrogen^a	36–6900
1G6-biotin	Affinity-purified monoclonal	Mouse IgG2b	Covance^b	SIG-39181
4G8	Affinity-purified monoclonal	Mouse IgG2b, κ	Covance^b	SIG-39220
6E10	Affinity-purified monoclonal	Mouse IgG1	Covance^b	SIG-39320
6E10-biotin	Affinity-purified monoclonal	Mouse IgG1	Covance^b	SIG-39340
82E1-biotin	Affinity-purified monoclonal	Mouse IgG1	IBL America (Minneapolis, MN)	10,326
A11	Rabbit serum	NA^c	Gift of Dr. Charles Glabe
22C11	Affinity-purified monoclonal	Mouse IgG1	EMD Millipore^d	MAB348
Anti-Protein A-biotin	Affinity-purified monoclonal	Mouse IgG1	Sigma-Aldrich^d	B-3150
Anti-α-tubulin	Affinity-purified monoclonal	Mouse IgG1	Sigma-Aldrich^d	T-5168

^anow Thermo Fisher Scientific (Waltham, MA); ^bnow Biolegend (San Diego, CA); ^cNA = not applicable; ^dnow MilliporeSigma (Burlington, MA)

ISSN: 1480-9222