Fig. 5
From: Effective Visualization and Easy Tracking of Extracellular Vesicles in Glioma Cells
Cellular uptake assay in HUVECs. Confocal imaging of EVs labeled with antibodies to CD63, TSG101, calnexin along with PKH67 dye. The uptake of CD63 (upper panel) and TSG101 antibodies (lower panel) labeled EVs along with PKH67 dye molecules were analysed in HUVECs after 6 h of incubation with EVs (a and b). Calnexin and PKH67 dual staining served as a negative control to ensure specificity of signal (c). EVs labeled with only secondary antibody and PKH67 dye served as negative control (d)