Fig. 2
From: Effective Visualization and Easy Tracking of Extracellular Vesicles in Glioma Cells
Characterization and visualization of EVs using confocal and STED microscope. Western blotting for different exosomal marker proteins like- TSG101, CD63, HSP70 and ER marker like- calnexin (a). Confocal and STED imaging of EVs labeled with TSG101 and CD63 antibodies and visualized using Alexa 594 species specific antibodies (b). Analyses and comparison of the size of EVs through confocal and STED microscopy (p*** < 0.0001) (c). Confocal imaging for HSP70 labeled EVs (d). dSTORM images of EVs stained with CD63 (red) and TSG101 (green) antibodies (e). Analyses of the size distribution of immune-labeled EVs by dSTORM Nanoimager (f)