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Fig. 4 | Biological Procedures Online

Fig. 4

From: A simple and reliable protocol for long-term culture of murine bone marrow stromal (mesenchymal) stem cells that retained their in vitro and in vivo stemness in long-term culture

Fig. 4

Increased the response of BMSCs-FS to osteogenic signaling factors. a, b Response of BMSCs-FS (p25) Vs ST2 cells to different osteogenic growth factors as measured by quantitative ALP activity. Cells were either cultured without osteogenic induction media (C, control), or induced to osteogenic lineage in the absence (I, induced) or the presence of 10 and 100 ng/mL of different osteogenic growth factors for 6 days. Representative images of ALP staining are shown. ALP measurements were normalized to cell number and represented as fold change over control non-induced cells. Data are mean ± SD of three independent experiments. (*p < 0.05, **p < 0.005, compared to ST2 cells with the same condition). Columns of the same group with different letters at each growth factor indicate significant differences according to Duncan’s multiple range tests at p < 0.05

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