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Fig. 1 | Biological Procedures Online

Fig. 1

From: A simple and reliable protocol for long-term culture of murine bone marrow stromal (mesenchymal) stem cells that retained their in vitro and in vivo stemness in long-term culture

Fig. 1

Long term culturing of primary mBMSCs using a combination of FS and bFGF. a Workflow of simple and reliable protocol used to culture and expand primary mBMSCs in long term. b Long term growth curves of primary isolated mBMSCs cultured by traditional method and three independent BMSCs-FS cell lines cultured by our new protocol in the presence of bFGF. c Phase contrast representative images (20x) of pBMSCs (p5) and three independent BMSCs-FS cell lines (at 70 PDL, p25). d FACS analysis of the expression of MSC surface markers by BMSCs-FS, p25. Values are mean ± SD of three independent experiments

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