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Table 1 Parameters of the experiments

From: Circular Estimate Method (CEM) - a Simple Method to Estimate Caenorhabditis elegans Culture Densities in Liquid Medium

Operator

NSS volume

Sample volume

ENS volume

OP 1

10 mL

250 μL

1.5 mL

 

8.01 ml

275 μl

1.65 mL

 

5.96 ml

300 μl

1.8 mL

 

3.76 mL

325 μL

1.95 mL

 

8.02 mL

350 μL

2.1 mL

 

5.52 mL

375 μL

2.25 mL

 

2.87 mL

400 μL

2.4 mL

 

6.67 mL

425 μL

2.55 mL

 

3.72 mL

450 μL

2.7 mL

 

5.77 mL

475 μL

2.85 mL

 

4.32 mL

500 μL

3 mL

OP 2

6.97 mL

300 μL

1.8 mL

 

6.03 mL

325 μL

1.95 mL

 

4.77 mL

375 μL

2.25 mL

 

7.77 mL

400 μL

2.4 mL

 

3.55 mL

425 μL

2.55 mL

 

4.87 mL

500 μL

3 mL

OP 3

8.05 mL

250 μL

1.5 mL

 

2.93 mL

275 μL

1.65 mL

 

6.15 mL

350 μL

2.1 mL

 

3.65 mL

450 μL

2.7 mL

 

3.5 mL

475 μL

2.85 mL

  1. First column: the operator (OP 1, OP 2 and OP 3) responsible for each experiment. Second column: The total stock volume used as a base for the experiment. Third column: The aliquot used as a sample for CEM. Fourth column: The total experimental nematode suspension (ENS) assessed in each experiment