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Fig. 8 | Biological Procedures Online

Fig. 8

From: Circular Estimate Method (CEM) - a Simple Method to Estimate Caenorhabditis elegans Culture Densities in Liquid Medium

Fig. 8

The sequence of steps in using ImageJ software. a Calibration of software. b Set of tools available for calibration. c The relation between pixels/mm and confirming of set scale. d Selection of oval/circular form. e Randomly placing the 1/10 counting circles. f Displacement of counting circles (black circles) and local reserved for homogenization (red circle at the centre). g Counting animals. h Selecting animals to be counted from those that are not (crossed animals). i Recording animal numbers for extrapolation to total NSS or ENS

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Biological Procedures Online

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