Table 5 DNA constructs used in this study
From: CRISPR/Cas9 Knockout Strategies to Ablate CCAT1 lncRNA Gene in Cancer Cells
Construct | Features | Application |
|---|---|---|
pX459_2 | hU6 promoter- sgRNA (downstream of CCAT1 Exon 1)-sgRNA scaffold-CAG promoter-Cas9-T2A- PuroR-bGH polyA | CRISPR Excision CRISPR du-HITI |
pX459_3 | hU6 promoter- sgRNA (upstream of CCAT1 Exon 1)-sgRNA scaffold-CAG promoter-Cas9-T2A- PuroR-bGH polyA | CRISPR Excision CRISPR du-HITI |
pX459_13 | hU6 promoter- sgRNA (CCAT1 Exon 2)-sgRNA scaffold-CAG promoter-Cas9-T2A- PuroR-bGH polyA | CRISPR HDR |
pHD_4317_CCAT1 E2 HAs | Exon 2 LHA-DsRed2-HSV TK polyA-CMV promoter –PuroR-IRES2-EGFP-SV40 polyA-Exon 2 RHA | CRISPR HDR |
pX460_11 | hU6 promoter-sgRNA (downstream of CCAT1 Exon 1) plus PAM-sgRNA scaffold-CAG promoter-EGFP-bGH polyA | CRISPR du-HITI |
pX461_11 | hU6 promoter-sgRNA (downstream of CCAT1 Exon 1) plus PAM-sgRNA scaffold-CAG promoter-PuroR-bGH polyA | CRISPR du-HITI |